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KMID : 1025520040460060903
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Journal of Animal Science and Technology
2004 Volume.46 No. 6 p.903 ~ p.908
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Characteristics and Frequencies of Alternative Initiation Codon(AIC) of mtDNA ND2 in Five Pig Breeds
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Han Sang-Hyun
Cho In-Cheol
Choi Y. L.
Lee Chong-Eon
Ko Moon-Suck
Kim Jae-Hwan
Seo Bo-Yeong
Lee Jung-Gyu
Jeon Jin-Tae
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Abstract
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Alternative initiation codon(AIC) has been reported in the mitochondrial genes in various mammalian species. We investigated the AIC of mitochondrial NADH dehydrogenase 2 gene(mtDNA ND2) in five pig breeds. Two kinds of initiation codons(ATA/ATT) showing different frequencies among tested pig breeds were used. While all Large White pigs had ATA as an initiator methionine codon, all Landrace pigs had ATT. The other breeds(Berkshire, Duroc and Hampshire) had both initiation codons with the ATA frequencies, 91.9, 21.3 and 60.0%, respectively. In the previous reports, all Chinese indigenous pig breeds were identified to have unique initiation codon ATA. Although the effect of AIC on the translation of mtDNA ND2 has not been studied in this study, AIC patterns in mtDNA ND2 will contribute to the maternity test using molecular markers in pig breeding.
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KEYWORD
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Alternative initiation codon, mtDNA, ND2, Breed-specific, Maternity test
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